Radiochemistry and radiopharmacy of the SPECT-tracer technetium- 99m -hexakis-2-methoxy-2-isobutyl isonitrile ( 99m Tc-Sestamibi).- Physics and radiation exposure.- Preparation of Tc-99m-sestamibi.- Parathyroid imaging.- Myocardial perfusion scintigraphy with 99mTc-MIBI.- 99m Tc-sestamibi scintimammography.- TC-99m-MIBI for thyroid Imaging.- Tc-99m sestamibi in miscellaneous tumors.- Oncologic applications of sestamibi: in vivo imaging of multi-drug resistance.