Part I: Molecular Tools and Technology for Yeast Engineering§§1. An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae§§Verena Siewers§§2. Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae§§Georg Hubmann, Johan M. Thevelein, and Elke Nevoigt§§3. Tools for Genetic Engineering of the Yeast Hansenula polymorpha§§Ruchi Saraya, Loknath Gidijala, Marten Veenhuis, and Ida J. van der Klei§§4. Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory§§Paola Branduardi, Laura Dato, and Danilo Porro§§5. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris§§Michael Felber, Harald Pichler, and Claudia Ruth§§6. Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains§§Andrea Camattari, Katrin Weinhandl, and Rama K. Gudiminchi§§7. Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries§§Joshua K. Michener and Christina D. Smolke§§8. Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology§§Kouichi Kuroda and Mitsuyoshi Ueda§§9. Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach§§Dariusz R. Kutyna, Antonio G. Cordente, and Cristian Varela§§10. Evolutionary Engineering of Yeast§§Ceren Alkm, Burcu Turanl-Y_ld_z, and Z. Petek Çakar§§11. Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains§§Oliver Spadiut, Christian Dietzsch, and Christoph Herwig§§Part II: Tools and Technologies for Investigation and Determination of Yeast Metabolic Features§§12. Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis§§Sónia Carneiro, Rui Pereira, and Isabel Rocha§§13. 13 C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case§§Pau Ferrer and Joan Albiol§§14. Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis§§Raphael B.M. Aggio§§15. QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast§§Thiago M. Pais, María R. Foulquié-Moreno, and Johan M. Thevelein§§Part III: Metabolic Models for Yeast Metabolic Engineering§§16. Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction, and Other Applications§§Sergio Bordel§§17. Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae§§Ana Rita Brochado and Kiran Raosaheb Patil§§Part IV: Patenting and Regulations§§18. Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace§§Z. Ying Li and Wolfram Meyer§